Genome-Resolved Metagenomic Analyses Reveal the Presence of a Putative Bacterial Endosymbiont in an Avian Nasal Mite (Rhinonyssidae; Mesostigmata).

Microorganisms

Department of Microbiology and Parasitology, Faculty of Pharmacy, Universidad de Sevilla, Calle San Fernando, 4, 41004 Sevilla, Spain.

Published: August 2021

Rhinonyssidae (Mesostigmata) is a family of nasal mites only found in birds. All species are hematophagous endoparasites, which may damage the nasal cavities of birds, and also could be potential reservoirs or vectors of other infections. However, the role of members of Rhinonyssidae as disease vectors in wild bird populations remains uninvestigated, with studies of the microbiomes of Rhinonyssidae being almost non-existent. In the nasal mite () from rock doves (), a previous study found evidence of a highly abundant putatively endosymbiotic bacteria from Class Alphaproteobacteria. Here, we expanded the sample size of this species (two different hosts- ten nasal mites from two independent samples per host), incorporated contamination controls, and increased sequencing depth in shotgun sequencing and genome-resolved metagenomic analyses. Our goal was to increase the information regarding this mite species and its putative endosymbiont. We obtained a metagenome assembled genome (MAG) that was estimated to be 98.1% complete and containing only 0.9% possible contamination. Moreover, the MAG has characteristics typical of endosymbionts (namely, small genome size an AT bias). Overall, our results support the presence of a potential endosymbiont, which is the first described for avian nasal mites to date, and improve the overall understanding of the microbiota inhabiting these mites.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398770PMC
http://dx.doi.org/10.3390/microorganisms9081734DOI Listing

Publication Analysis

Top Keywords

nasal mites
12
genome-resolved metagenomic
8
metagenomic analyses
8
avian nasal
8
nasal mite
8
rhinonyssidae mesostigmata
8
nasal
6
analyses reveal
4
reveal presence
4
presence putative
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!