AI Article Synopsis
- - The study focuses on an oleaginous yeast known for its ability to produce triacylglycerol (TAG), which is important for biodiesel production, and investigates the regulation of TAG synthesis by isolating mutants with reduced lipid production.
- - A mutant strain named sr22 was identified through a specific screening method, and it showed lower expression levels of genes related to citrate-mediated acyl-CoA synthesis compared to the wild-type yeast, indicating a link between gene expression and TAG synthesis efficiency.
- - The research further demonstrates that ATP-citrate lyase, an enzyme crucial for the citrate-mediated acyl-CoA synthesis pathway, is necessary for both cell growth and TAG production when the yeast is grown on glucose, suggesting potential
Article Abstract
The oleaginous yeast is an excellent producer of triacylglycerol (TAG) as a feedstock for biodiesel production. To understand the regulation of TAG synthesis, we attempted to isolate mutants with decreased lipid productivity and analyze the expression of TAG synthesis-related genes in this study. A mutant with greatly decreased lipid productivity, sr22, was obtained by an effective screening method using Percoll density gradient centrifugation. The expression of citrate-mediated acyl-CoA synthesis-related genes (, , , , and ) was decreased in the sr22 mutant compared with that of the wild-type strain. Together with a notion that mutants with increased lipid productivities had increased gene expression, there was a correlation between the expression of these genes and TAG synthesis. To clarify the importance of citrate-mediated acyl-CoA synthesis pathway on TAG synthesis, we also constructed a strain with no ATP-citrate lyase responsible for the first reaction of citrate-mediated acyl-CoA synthesis and investigated the importance of ATP-citrate lyase on TAG synthesis. The ATP-citrate lyase was required for the promotion of cell growth and TAG synthesis in a glucose medium. This study may provide opportunities for the development of an efficient TAG synthesis for biodiesel production.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400019 | PMC |
| http://dx.doi.org/10.3390/microorganisms9081693 | DOI Listing |
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