(shiitake mushrooms) is heavily affected by the infection of , causing yield loss and decreases quality in shiitake mushrooms. The selection and breeding of fungal-resistant species are an important approach to protecting from infection. Herein, a highly resistant strain (Y3334) and a susceptible strain (Y55) were obtained by using a resistance evaluation test. Transcriptome analyses and qRT-PCR detection showed that the expression level of () was strongly induced in response to infection in the resistant Y3334. Then, silenced and -overexpression transformants were obtained. Overexpression of resulted in resistance to . Compared with the parent strain Y3334, silenced transformants had reduced resistance relative to Additionally, the TLP1 protein (Y3334) exhibited significant antifungal activity against . These findings suggest that overexpression of is a major mechanism for the resistance of to The molecular basis provides a theoretical basis for the breeding of resistant strains and can eventually contribute to the mushroom cultivation industry and human health.
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| http://dx.doi.org/10.3390/life11080863 | DOI Listing |
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