Antimicrobial resistance (AMR) is a worldwide health problem affecting humans, animals, and the environment within the framework of the "One Health" concept. The aim of our study was to evaluate the prevalence of pathogenic strains of the species (), their AMR profile, and biofilm-forming potential. The isolated strains from three swine faeces and free lagoons (ISO 16654:2001/Amd 1:2017) were confirmed using Phoenix M50 and 16S rDNA PCR. The antibiotic sensitivity to 34 clinically applied antibiotics was determined by Phoenix M50 and the disc diffusion method, according to the protocols of the CLSI and EUCAST. We confirmed the presence of 16 isolates, of which 87.5% were multi-drug-resistant and 31.25% performed strong biofilms. The possibility for the carrying and transmission of antibiotic-resistance genes to quinolones (), aminoglycosides ((3)), β-lactamase-producing plasmid genes C, and SHV/TEM was investigated. We confirmed the carrying of SHV/TEM in one and C in seven isolates. The strains were negative for the virulence genes (ETEC (LT, STa, and F4), EPEC (), and STEC/VTEC ( and 2all)). The results should contribute to the development of effective measures for limitation and control on the use of antibiotics, which is a key point in the WHO action plan.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8388900 | PMC |
| http://dx.doi.org/10.3390/antibiotics10080940 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
First molecular characterisation of in a pig carcass condemned during routine meat inspection in Poland.
J Vet Res
December 2024
Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, 24-100 Puławy, Poland.
Introduction: This article presents the fourth detection of macroscopic cystic lesions due to sarcocystosis in domestic pigs during routine meat inspection worldwide, and the first molecular detection of in a domestic pig in Poland. Pigs can become intermediate hosts for by accidental ingestion of oocysts or sporocysts present in food or water contaminated by the faeces of canids (definitive hosts).
Material And Methods: The affected swine showed no clinical symptoms such as weight loss, dermatitis or dyspnoea suggesting sarcocystosis.
Preliminary evaluation of ex vivo and in vivo skin permeability of aromatic amines.
PLoS One
January 2025
Research Center for Chemical Information and Management, National Institute of Occupational Safety and Health, Kawasaki, Japan.
A potential link has been reported between skin exposure to aromatic amines, such as ortho-toluidine (OT) and 3,3'-dichloro-4,4'-diaminodiphenylmethane (MOCA), and bladder cancer cases observed in Japanese chemical factories. To evaluate this association, we explored the permeability of OT and MOCA through pig skin and investigated the subsequent changes in plasma and urine concentrations in rats following percutaneous exposure. Employing Yucatan micropig skin, we first executed a permeability test by affixing the skin to a diffusion cell and applying 14C-labeled OT or MOCA.
View Article and Find Full Text PDFMolecular Characterization and Pathogenicity Analysis of Porcine Rotavirus A.
Viruses
November 2024
National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Porcine rotavirus A (RVA) is one of the major etiological agents of diarrhea in piglets and constitutes a significant threat to the swine industry. A molecular epidemiological investigation was conducted on 2422 diarrhea samples from Chinese pig farms to enhance our understanding of the molecular epidemiology and evolutionary diversity of RVA. The findings revealed an average RVA positivity rate of 42% (943/2422), and the study included data from 26 provinces, primarily in the eastern, southern and southwestern regions.
View Article and Find Full Text PDFDevelopment of a Quadruplex RT-qPCR for the Detection of Porcine Astrovirus, Porcine Sapovirus, Porcine Norovirus, and Porcine Rotavirus A.
Pathogens
November 2024
College of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Porcine astrovirus (PoAstV), porcine sapovirus (PoSaV), porcine norovirus (PoNoV), and porcine rotavirus A (PoRVA) are newly discovered important porcine diarrhea viruses with a wide range of hosts and zoonotic potential, and their co-infections are often found in pig herds. In this study, the specific primers and probes were designed targeting the ORF1 gene of PoAstV, PoSaV, and PoNoV, and the VP6 gene of PoRVA. The recombinant standard plasmids were constructed, the reaction conditions (concentration of primers and probes, annealing temperature, and reaction cycle) were optimized, and the specificity, sensitivity, and reproducibility were analyzed to establish a quadruplex real-time quantitative RT-PCR (RT-qPCR) assay for the detection of these four diarrheal viruses.
View Article and Find Full Text PDFFirst Detection of a Novel Posavirus 2 Strain Identified from Pigs in China.
Pathogens
November 2024
Sichuan Provincial Center for Animal Disease Control and Prevention, Wuhou District, Chengdu 610041, China.
Porcine stool-associated RNA virus (Posavirus) is an unclassified virus with sequence similarity to viruses in the order of Picornaviridae. In China, lineage 1 Posavirus (Posavirus 1) has been circulating in the field since its initial emergence in 2014 however no other lineages have been reported. To investigate the genetic diversity of Posavirus in China, 1200 diarrheic samples were collected from pigs in China.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!