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Department of Medicine, New Jersey Medical School, Rutgers - The State University of New Jersey;
Fungi infect humans when environmental spores are inhaled into the lungs. The lung is a heterogeneous organ. Conducting airways, including bronchi and bronchioles, branch until terminating in the alveolar airspace where gas exchange occurs.
View Article and Find Full Text PDFSimple Isolation of Human Bone Marrow Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells.
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Center for Stem Cell Research and Development (PEDI-STEM), Hacettepe University, Ankara, Turkey.
Bone marrow adipose tissue (BMAT) has garnered significant attention due to its critical roles in leukemia pathogenesis, cancer metastasis, and bone marrow failure. BMAT is a metabolically active, distinct tissue that differs from other fat depots. Marrow adipocytes, closely interacting with hematopoietic stem/progenitor cells and osteoblasts, play a pivotal role in regulating their functions.
View Article and Find Full Text PDFTrehalose Cryopreservation of Human Mesenchymal Stem Cells from Cord Tissue.
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Adequate hypothermic storage of human mesenchymal stem cells (hMSCs) is of fundamental importance since they have been explored in several regenerative medicine initiatives. However, the actual clinical application of hMSCs necessitates hypothermic storage for long periods, a process that requires the use of non-toxic and efficient cryo-reagents capable of maintaining high viability and differentiating properties after thawing. Current cryopreservation methods are based on cryoprotectant agents (CPAs) containing dimethylsulphoxide (DMSO), which have been shown to be toxic for clinical applications.
View Article and Find Full Text PDFRecovery effects of the long-term cryopreserved Anammox sludge by adjusting the sludge amount.
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Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Materials and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou, People's Republic of China.
Anammox process was one of the most promising nitrogen removal technologies. This study investigated the recovery performance of Anammox sludge after 83 days of cryopreservation in two reactors (R1 and R2). Reactor R1 utilized Anammox sludge pretreated with low-substrate simulated wastewater prior to long-term cryopreservation, and successful recovery was achieved by discharging sludge under ammonia nitrogen concentrations of 100 mg/L.
View Article and Find Full Text PDFCryopreservation of Human Adult Ventricular Tissue for the Preparation of Viable Myocardial Slices.
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Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, Netherlands.
Article Synopsis
- * A new cryopreservation method has been developed, allowing for the preparation of LMS from human myocardium at a later time, overcoming the limitations of immediate slice preparation after tissue extraction.
- * Results show that cryopreserved cardiac tissue maintains structural integrity and viability comparable to fresh tissue, enabling longer and more complex experiments in cardiac research.
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