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Activity-guided isolation of cholinesterase inhibitors quercetin, rutin and kaempferol from fruit. | LitMetric

Activity-guided isolation of cholinesterase inhibitors quercetin, rutin and kaempferol from fruit.

Z Naturforsch C J Biosci

Institut für Pharmazeutische Biologie, Universität des Saarlandes, Campus C2 3, 66123 Saarbrücken, Germany.

Published: March 2020

AI Article Synopsis

  • * A new method was developed to purify these cholinesterase inhibitors from a water extract of fruit, involving ultrafiltration and chromatographic techniques.
  • * The researchers identified key inhibitors such as kaempferol, quercetin, and rutin in peach fruit, confirming their structures through spectroscopy and measuring their anti-acetylcholinesterase activity at each purification stage.

Article Abstract

fruit is a source of anti-cholinesterase agents and led to an increase of acetylcholine levels in brain tissue as a usefull tool in the Alzheimer's disease therapy. This study aimed to propose a convenient method for the purification of cholinesterase inhibitors from water extract. Water extract from the fruit was ultrafiltered (0.2 μm→5 kDa→3 kDa) followed by preparative chromatography (Sephadex LH-20, high pressure C18) and high pressure analytical C18 chromatography. The chemical structures of inhibitors were confirmed using infrared and H-NMR spectroscopies. The anti-acetylcholinesterase activity was measured using the colorimetric method in fractions obtained after each stage of purification. Polyphenolic cholinesterase inhibitors identified in peach fruit were kaempferol, quercetin and quercetin-3-O-rhamnoglucoside (rutin). The relatively fast purification procedure elaborated in this work can be adopted for the isolation of phenolic cholinesterase inhibitors from fruit extracts related to fruit.

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Source
http://dx.doi.org/10.1515/znc-2019-0079DOI Listing

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