Background: Dopamine is one of the main mediators capable regulate the neuroimmune interaction and is involved in multiple sclerosis (MS) pathogenesis.
Objective: The aim of this study was to clarify the role of dopamine and its receptors in modulation of Th17-cells in MS.
Methods: 34 relapsing-remitting MS patients and 23 healthy subjects were examined. To assess the effect of dopamine on Th17-cells, CD4+ T-cells were cultured in the presence of dopamine and antagonist or agonist of D- or D-like dopaminergic receptors and stimulated with anti-CD3/CD28- microbeads. The levels of cytokines in supernatants were assessed by ELISA.
Results: Production of interleukin-17 (IL-17), interferon-γ (IFN-γ), granulocyte-colony stimulating factor (GM-CSF), and IL-21 by CD4+ T-cells as well as dopamine were comparable between the groups. Dopamine suppressed cytokine secretion by activated СD4+ T-cells in both groups. Blockade of D1-like dopaminergic receptor with a specific antagonist SCH23390 did not affect dopaminemediated cytokine suppression. In contrast, blockade of D-like dopaminergic receptor by sulpiride decreased dopamine's inhibitory effect on IL-17 secretion in both groups and GM-CSF and IL-21 production in MS patients. Blockade of D-like dopaminergic receptor directly inhibited IL-17, IFN- γ, GM-CSF in both groups and IL-21 production in healthy subjects, while blockade of D-like dopaminergic receptor had no effect on cytokine secretion. Finally, activation of D-like dopaminergic receptor with a specific agonist quinpirole decreased cytokine production in both groups.
Conclusion: These data suggest an inhibitory role of dopamine on Th17-cells in MS, which could be mediated by the activation of the D-like dopaminergic receptor.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881083 | PMC |
| http://dx.doi.org/10.2174/1570159X19666210823103859 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Activation of Dopamine D Receptor Subtypes Inhibits the Neurogenic Systemic Vasodilation Induced by Stimulation of the Perivascular CGRPergic Discharge.
ACS Chem Neurosci
August 2019
Department of Pharmacobiology , Cinvestav-Coapa , Czda. Tenorios 235, Col. Granjas-Coapa , Deleg. Tlalpan, 14330 Mexico City , Mexico.
The sensory nervous system controls cardiovascular homeostasis via capsaicin-sensitive neurons that release calcitonin gene-related peptide (CGRP), which subsequently activates CGRP receptors. How this perivascular CGRPergic discharge is modulated, nevertheless, remains unclear. In pithed rats, systemic vasodilation induced by CGRPergic discharge stimulation results in diastolic blood pressure (BP) decrements that are inhibited by the dopamine D-like receptor agonist quinpirole.
View Article and Find Full Text PDFDopamine-induced functional activation of Gα mediated by dopamine D-like receptor in rat cerebral cortical membranes.
J Recept Signal Transduct Res
February 2019
a Department of Psychiatry, Faculty of Medicine , Saitama Medical University, Saitama , Japan.
Although multiple roles of dopamine through D-like (D and D) and D-like (D, D, and D) receptors are initiated primarily through stimulation or inhibition of adenylyl cyclase via G or G, respectively, there have been many reports indicating diverse signaling mechanisms that involve alternative G protein coupling. In this study, dopamine-induced Gα activation in rat brain membranes was investigated. Agonist-induced Gα activation was assessed by increase in guanosine-5'--(3-[S]thio)triphosphate ([S]GTPγS) binding to Gα determined by [S]GTPγS binding/immunoprecipitation assay in rat brain membranes.
View Article and Find Full Text PDFFunctional Characterization of the Prejunctional Receptors Mediating the Inhibition by Ergotamine of the Rat Perivascular Sensory Peptidergic Drive.
ACS Chem Neurosci
July 2019
Departamento de Farmacobiología , Cinvestav-Coapa , Czda. de los Tenorios 235, Col. Granjas-Coapa, Deleg. Tlalpan , 14330 México D.F. , Mexico.
Calcitonin gene-related peptide (α-CGRP) released from perivascular sensory nerves induces decreases in diastolic blood pressure (DBP). Experimentally, this can be shown by spinal thoracic (T-T) electrical stimulation of these afferent fibers. Because ergotamine inhibits these neurogenic vascular responses and displays affinity for monoaminergic receptors that inhibit neurotransmitter release, we investigated whether this ergotamine-induced inhibition results from activation of serotonin 5-HT, dopamine D-like, and α-adrenergic receptors.
View Article and Find Full Text PDFIdentification and characterization of a novel amphioxus dopamine D-like receptor.
J Neurochem
October 2009
The Inositide Laboratory, The Babraham Institute, Cambridge, UK.
Dopamine receptors function to control many aspects of motor control and other forms of behaviour in both vertebrates and invertebrates. They can be divided into two main groups (D(1) and D(2)) based on sequence similarity, ligand affinity and effector coupling. However, little is known about the pharmacology and functionality of dopamine receptors in the deuterostomian invertebrates, such as the cephalochordate amphioxus (Branchiostoma floridae) which has recently been placed as the most basal of all the chordates.
View Article and Find Full Text PDFChanges of dopamine receptors in mice with olivocerebellar degeneration.
Prague Med Rep
September 2007
Institute of Physiology of the First Faculty of Medicine, Charles University, Prague, Czech Republic.
Lurcher mutants are mice with functional mutation in the 82 glutamate receptor (GluRdelta2) that is predominantly expressed in cerebellar Purkinje cells and plays a crucial role in cerebellar functions. These mice display ataxia and impaired motor-related learning tasks. In order to elucidate the role of dopaminergic receptor system in coping with mutation in delta2 glutamate receptor the behavioral effect (spatial learning) of D1 dopamine receptor activation and inhibition and changes in D1-like and D2-like dopamine receptors in striatum, hippocampus and cerebellum in C57BI/7 and C3H Lurcher mutants and wild type mice were studied.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!