AI Article Synopsis
- Quorum sensing (QS) in bacteria, specifically through acyl-homoserine lactones (AHLs), is crucial for plant-bacteria interactions, highlighted by the study of the plant-growth-promoting rhizobacterium (PGPR) M15 isolated from rice roots.
- Researchers identified a QS system in M15's plasmid and discovered five AHL analogues using LC-MS, with the most prevalent being a specific AHL analogue confirmed through NMR.
- A mutant strain lacking AHL production showed significantly reduced growth, exopolysaccharide production, biofilm formation, and motility, paving the way for deeper exploration into QS mechanisms relating to M15 and its interaction with plants.
Article Abstract
Quorum sensing (QS), usually performed by -acyl-homoserine lactones (AHLs) in Gram-staining-negative bacteria, plays an important role in plant-bacteria interactions. M15 is a plant-growth-promoting rhizobacterium (PGPR) isolated from rice roots. In this study, we found a QS system in the endogenous plasmid of M15 and detected the activity of AHLs by a bioassay method. We identified five AHL analogues in M15 using liquid chromatography-tandem mass spectrometry (LC-MS). The most dominant AHL analogue was -(3R-hydroxy-7--tetradecanoyl)-l-homoserine lactone according to nuclear magnetic resonance (NMR) and Mosher's reactions. Furthermore, the mutant abolished AHL production and significantly decreased growth, exopolysaccharide (EPS) production, biofilm formation, and motility compared to the wild-type strain. These results lay the foundation for further investigating the QS regulation mechanism and signal pathway of M15 and its interactions with the host plant.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.1c03895 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!