The germline genomes of ciliated protists are replete with "junk" DNA insertions that need to be removed for gene expression. Unlike introns, these are spliced as DNA. What is their source, and why are they so abundant? A new study in PLOS Biology supports a classic model of transposon origins.
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http://dx.doi.org/10.1371/journal.pbio.3001354 | DOI Listing |
Folia Microbiol (Praha)
April 2024
Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 76, 323 00, Pilsen, Czech Republic.
PLoS Biol
August 2021
Departments of Biochemistry & Molecular Biophysics and Biological Sciences, Columbia University, New York, New York, United States of America.
The germline genomes of ciliated protists are replete with "junk" DNA insertions that need to be removed for gene expression. Unlike introns, these are spliced as DNA. What is their source, and why are they so abundant? A new study in PLOS Biology supports a classic model of transposon origins.
View Article and Find Full Text PDFJ Environ Monit
July 2012
Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195-2700, USA.
Antibiotic resistance genes (ARGs), in association with antibiotic resistant bacteria (ARB), have been identified as widespread contaminants of treated drinking waters and wastewaters. As a consequence, concerns have been raised that ARB or ARG transport between aquatic compartments may enhance the spread of antibiotic resistance amongst non-resistant bacterial communities by means of horizontal gene transfer processes. Most often, discussion of horizontal gene transfer focuses on the probable role of conjugative plasmid or transposon exchange, which requires live ARB donor cells.
View Article and Find Full Text PDFPLoS One
September 2012
Institute of Microbiology, ETH Zürich, Zürich, Switzerland.
Background: Enteric pathogens need to grow efficiently in the gut lumen in order to cause disease and ensure transmission. The interior of the gut forms a complex environment comprising the mucosal surface area and the inner gut lumen with epithelial cell debris and food particles. Recruitment of neutrophils to the intestinal lumen is a hallmark of non-typhoidal Salmonella enterica infections in humans.
View Article and Find Full Text PDFWhen a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein's activity or fluorescence within a transfected cell population is approximately proportional to the steady-state mRNA level.
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