CTCF belongs to a large family of transcription factors with clusters of C2H2-type zinc finger domains (C2H2 proteins) and is a main architectural protein in mammals. Human CTCF has a homodimerizing unstructured domain at the N-terminus which is involved in long-distance interactions. To test the presence of similar N-terminal domains in other human C2H2 proteins, a yeast two-hybrid system was used. In total, the ability of unstructured N-terminal domains to homodimerize was investigated for six human C2H2 proteins with an expression profile similar to CTCF. The data indicate the lack of the homodimerization ability of these domains. On the other hand, three C2H2 proteins containing the structured domain DUF3669 at the N-terminus demonstrated homo- and heterodimerization activity.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382608PMC
http://dx.doi.org/10.1134/S1607672921040050DOI Listing

Publication Analysis

Top Keywords

c2h2 proteins
16
zinc finger
8
n-terminal domains
8
human c2h2
8
proteins
5
study n-terminal
4
n-terminal domain
4
domain homodimerization
4
human
4
homodimerization human
4

Similar Publications

KLF6 silencing attenuates MCAO-induced brain injury and cognitive dysfunction via targeting ferroptosis and activating the Nrf2/HO-1 pathway.

Hum Exp Toxicol

December 2024

Department of neurology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China. Hubei Sizhen Laboratory, Affiliated Hospital of Hubei University of Chinese Medicine, Wuhan, China.

Introduction: The incidence of cerebral ischemia-reperfusion injury (I/R) is complex which seriously threatens the life safety of patients. Neither its prevention nor its treatment has been successful so far. Proteins that bind to DNA and belong to the C2/H2 zinc finger family are known as Krüppel-like factors (KLFs).

View Article and Find Full Text PDF

Context: Most of the loss-of-function mutations described in children with central precocious puberty (CPP) is located into the coding regions of MKRN3 or DLK1 genes. Notably, potential abnormalities in the regulatory regions of these CPP-genes are rarely explored.

Objective: To search for pathogenic variants in the regulatory regions of MKRN3 and DLK1 genes in patients with familial or idiopathic CPP.

View Article and Find Full Text PDF

Melatonin (MT) serves an indispensable function in plant development and their response to abiotic stress. Although numerous drought-tolerance genes have been ascertained in wheat, further investigation into the molecular pathways controlling drought stress tolerance remains necessary. In this investigation, it was observed that MT treatment markedly enhanced drought resistance in wheat by diminishing malondialdehyde (MDA) levels and augmenting the activity of antioxidant enzymes POD, APX, and CAT compared to untreated control plants.

View Article and Find Full Text PDF

Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets.

Planta

December 2024

Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531 (2000), Rosario, Argentina.

PpZAT12, a transcription factor differentially expressed in peach varieties with distinct susceptibility tochilling injury (CI), is a potential candidate gene for CI tolerance by regulating several identified gene targets. ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a heat treatment applied before postharvest cold storage for reducing chilling injury (CI) symptoms.

View Article and Find Full Text PDF

Harnessing light-harvesting chlorophyll a/b-binding proteins for multiple abiotic stress tolerance in Chlamydomonas reinhardtii: Insights from genomic and physiological analysis.

Physiol Plant

December 2024

Guangdong Key Laboratory of Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Technology Research Center for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.

Light-harvesting chlorophyll a/b-binding proteins (LHC) of photosystem II perform key functions in various processes, e.g., photosynthesis, development, and abiotic stress responses.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!