Rapid removal of chloramphenicol via the synergy of Geobacter and metal oxide nanoparticles.

The wide use of chloramphenicol and its residues in the environments are an increasing threat to human beings. Electroactive microorganisms were proven with the ability of biodegradation of chloramphenicol, but the removal rate and efficiency need to be improved. In this study, a model electricigens, Geobacter metallireducens, was supplied with and FeO and MnO nanoparticles. Five times higher chloramphenicol removal rate (0.71 d) and two times higher chloramphenicol removal efficiency (100%) was achieved. FeO and MnO nanoparticles highly increased the current density and NADH-quinone oxidoreductase expression. FeO nanoparticles enhanced the expression of alcohol dehydrogenase and c-type cytochrome, while MnO nanoparticles increased the transcription of pyruvate dehydrogenase and Type IV pili assembly genes. Chloramphenicol was reduced to a type of dichlorination reducing product named CPD3 which is a benzene ring containing compound. Collectively, FeO and MnO nanoparticles increased the chloramphenicol removal capacity in MFCs by enhancing electron transfer efficiency. This study provides new enhancing strategies for the bioremediation of chloramphenicol in the environments.