AI Article Synopsis
- The COVID-19 pandemic prompted a surge in antibody tests for SARS-CoV-2, leading to an evaluation of twelve laboratory and six point-of-care immunoassays across Canada.
- There was significant variation in test performance, highlighting the need for an orthogonal testing strategy to enhance accuracy and consistency in results nationwide.
- Manual tests had lower specificity and higher variability than automated tests, raising concerns about their effectiveness for large-scale monitoring, ultimately guiding public health decisions in Canada.
Article Abstract
The COVID-19 pandemic has led to the influx of immunoassays for the detection of antibodies towards severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into the global market. The Canadian Public Health Laboratory Network Serology Task Force undertook a nationwide evaluation of twelve laboratory and 6 point-of-care based commercial serological assays for the detection of SARS-CoV-2 antibodies. We determined that there was considerable variability in the performance of individual tests and that an orthogonal testing algorithm should be prioritized to maximize the accuracy and comparability of results across the country. The manual enzyme immunoassays and point-of-care tests evaluated had lower specificity and increased coefficients of variation compared to automated enzyme immunoassays platforms putting into question their utility for large-scale sero-surveillance. Overall, the data presented here provide a comprehensive approach for applying accurate serological assays for longitudinal sero-surveillance and vaccine trials while informing Canadian public health policy.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377389 | PMC |
| http://dx.doi.org/10.1016/j.diagmicrobio.2021.115412 | DOI Listing |
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