The aim of this study was to assess the diagnostic accuracy of digital periapical radiography for the detection of the second mesiobuccal (MB2) canal when the adjacent canals are unfilled or filled with gutta percha. Forty human maxillary first molars were selected, 20 of which had an MB2 canal. The presence or absence of the MB2 canal was confirmed using microcomputed tomography. Digital periapical radiographs of each tooth were obtained from 3 horizontal angles before and after the adjacent root canals were filled with gutta percha. Three experienced oral radiologists evaluated all images and indicated the presence or absence of the MB2 canal using a confidence scale of 5 points. To assess intraobserver and interobserver agreement, weighted kappa coefficients were calculated. The diagnostic test (sensitivity, specificity, and accuracy) and area under the receiver operating characteristic curve (A) values were calculated and compared between groups (n = 40): radiographs of the first molars with unfilled adjacent canals and radiographs of the same teeth with filled adjacent canals. For all observers, intraobserver reproducibility ranged from substantial (0.70) to almost perfect (0.86), and interobserver reproducibility ranged from fair (0.39) to substantial (0.67). Similar values of specificity and A for the detection of the MB2 canal were observed with unfilled and filled adjacent canals. The sensitivity and accuracy were slightly greater when the adjacent root canals were filled with gutta percha. Detection of the MB2 canal on digital periapical radiographs can be challenging even when obtained at varying horizontal angles, and the presence of gutta percha does not influence the detection.
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