AI Article Synopsis
- Carbapenem-resistant bacteria are becoming a major concern in healthcare, and this study focuses on an outbreak of ertapenem-resistant strains in a Croatian hospital between 2012 and 2014.
- The research investigated the genetic relationships among these strains and the mechanisms causing their resistance to antibiotics, finding a link to the expression of certain porin channels.
- The results highlighted that most of the resistant isolates were genetically similar and suggested that these mutants could lead to further outbreaks if not managed carefully.
Article Abstract
Introduction: Carbapenem-resistant is an emerging healthcare-associated pathogen with dynamic molecular epidemiology. This study presents a retrospective analysis of the distribution and antibiotic resistance patterns of ertapenem-resistant ESBL-producing strains recovered during an outbreak from 2012 to 2014 in a Croatian University hospital.
Methods: We aimed to estimate genetic relatedness of clinical isolates and underlying mechanisms that conferred the ertapenem-resistant phenotype.
Results: Expression analysis of genes involved in the antibiotic resistance showed reduced expression of major non-selective porin channel OmpK35. Reduced expression of OmpK36 porin channel in isolates resistant to at least one more carbapenem, apart from the ertapenem, was found to a lesser degree. Pulsed-field gel electrophoresis analysis of genomic DNA revealed that almost all isolates belonged to the same genetic clone.
Conclusions: Caution regarding ertapenem-resistant, carbapenemase-negative porin-deficient mutants of is required as they are widespread, and under selective pressure this could result in a local clonal outbreak.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373410 | PMC |
| http://dx.doi.org/10.18683/germs.2021.1257 | DOI Listing |
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Article Synopsis
- Carbapenem-resistant bacteria are becoming a major concern in healthcare, and this study focuses on an outbreak of ertapenem-resistant strains in a Croatian hospital between 2012 and 2014.
- The research investigated the genetic relationships among these strains and the mechanisms causing their resistance to antibiotics, finding a link to the expression of certain porin channels.
- The results highlighted that most of the resistant isolates were genetically similar and suggested that these mutants could lead to further outbreaks if not managed carefully.
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