polysaccharides (EUP) can regulate the immunity of macrophages, but the functional status of macrophages is related to osteoarthritis and synovial inflammation. The purpose of this study is to explore whether EUP has the effect of inhibiting osteoarthritis and its possible mechanism. MTT test was used to evaluate the appropriate concentration of EUP and real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to detect the effect of EUP on gene expression in RAW 264.7 cells. The osteoarthritis model was constructed by the anterior cruciate ligament transection (ACLT) in the rabbits. These rabbits were divided into three groups, sham operation group, OA group, and EUP group. The changes in articular cartilage were detected by gross observation and histological staining, and Micro-CT tested subchondral bone. Finally, the changes of macrophages in synovial tissue were studied by immunohistochemistry. The results showed that EUP at the concentration of 50ug/mL and 100ug/mL were beneficial to the proliferation of macrophages. The qPCR results indicated that EUP inhibited the expression of inflammation-related genes IL-6, IL-18 and IL-1β, and promoted the expression of osteogenic and cartilage-related genes BMP-6, Arg-1 and transforming growth factor beta (TGF-β). The results of experiments suggested that the degree of destruction of articular cartilage in the EUP group was significantly reduced, and the Osteoarthritis Research Society International (OARSI) score was significantly reduced. Compared with the OA group, the subchondral cancellous bone density of the EUP group increased, the number and thickness of trabecular bone increased, and the separation of trabecular bone decreased. Synovial macrophage immunohistochemistry results manifested that EUP, on the one hand, reduced M1 polarized macrophages, on the other hand, accumulated M2 polarized macrophages. EUP can promote articular cartilage repair and subchondral bone reconstruction. The regulation of the polarization state of macrophages may be one of its mechanisms to delay the progression of osteoarthritis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377595 | PMC |
| http://dx.doi.org/10.3389/fphar.2021.730557 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Impact of the presence of a family member with dementia on the prevalence of depression: a comparison based on household income level.
Int J Equity Health
December 2024
Department of Preventive and Occupational & Environmental Medicine, Schools of Medicine, Pusan National University, 49 Pusan Daehak-ro, Mulgeum-eup, Yangsan-si, Yangsan, 50612, Gyeongsangnam-do, Republic of Korea.
Background: Families caring for patients with dementia are more vulnerable to depression. This cross-sectional study compared differences in socioeconomic status and gender related to depression among families of patients with dementia and identified associated factors.
Methods: Inequality in depression according to household income level among families of patients with dementia was assessed using the Korean Community Health Survey, which included a sample of over 200,000 participants.
Allelic and haplotype diversity of 12 X-chromosomal short tandem repeats in Koreans, with an analysis of anomalous profiles.
Leg Med (Tokyo)
December 2024
Forensic DNA Section, National Forensic Service Jeju Branch, 221, Cheomdan-ro, Jeju-si, Jeju-do 63309, Republic of Korea; Department of Forensic Medicine, Seoul National University College of Medicine, 103, Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea. Electronic address:
Owing to the unique inheritance pattern of the X chromosome, X-chromosomal short tandem repeat (X-STR) analysis represents a valuable tool in forensic DNA examination-particularly in complex kinship cases, missing person investigations, and disaster victim identification. We analyzed buccal swabs from 429 unrelated Korean males for forensic statistical parameters of 12 X-STRs. Among the 427 individuals analyzed (2 were excluded), DXS10135 was the most informative marker (polymorphism information content [PIC] = 0.
View Article and Find Full Text PDFWhole-Body and Segmental Phase Angles and Cognitive Function in the Older Korean Population: Cross-Sectional Analysis.
JMIR Public Health Surveill
December 2024
Biomedical Research Institute, Pusan National University Hospital, 179 Gudeok-ro, Seo-gu, Busan, 49241, Republic of Korea.
Background: Recently, the phase angle (PhA) has emerged as an essential indicator of cellular health. Most studies have examined its association with physiological conditions, such as sarcopenia, frailty, and physical function, in older populations. Simultaneously, growing attention is being paid to the clinical relevance of segmental PhAs for future applications.
View Article and Find Full Text PDFChemoselective Deprotection of Symmetrically Diprotected Resorcinol -Methyliminodiacetate (MIDA) Boronates Using the MIDA Boronate Moiety as a Blocking Group: Synthesis of Monoprotected Resorcinol MIDA Boronates.
Org Lett
December 2024
Department of Chemistry, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
A novel protocol for the synthesis of monoprotected resorcinol -methyliminodiacetate (MIDA) boronates was developed via the chemoselective deprotection of diprotected resorcinol MIDA derivatives with identical protecting groups, utilizing the MIDA boronate moiety as a blocking group for deprotection. This protocol exhibited a broad substrate scope, and the resulting MIDA boronates were readily isolated by simple filtration from the reaction mixture. Furthermore, the utility of this protocol was demonstrated by converting the resulting MIDA boronates into value-added chemicals containing resorcinol scaffolds.
View Article and Find Full Text PDFTransformative characteristics of aquatic and soil organic matter in a constructed wetland consisting of Acorus, Nuphar, and Typha ponds.
Sci Total Environ
December 2024
Department of Environmental Engineering, College of Art, Culture, and Engineering, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do 24341, Republic of Korea; Department of Integrated Energy and Infrasystem, Kangwon National University, Kangwondaehak-gil, 1, Chuncheon-si, Gangwon-do 24341, Republic of Korea. Electronic address:
Article Synopsis
- The study explored how dissolved organic matter (DOM) transforms in constructed wetlands (CWs) and its interaction with soil organic matter (SOM) during various treatment stages.
- Through methods like water quality assessments and molecular analyses, findings showed that anaerobic decomposition in the Typha pond significantly increased dissolved organic carbon (DOC) levels, indicating the role of microbial activity.
- The research highlighted that DOM in CWs interacts with organic fractions from both water and soil, influencing water quality improvement and offering insights for better CW design and management for wastewater treatment.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!