Diagnosis of osteoarticular tuberculosis (OATB) is quite challenging and there is an urgent need to design a prompt and precise diagnostic test. We developed a multi-targeted loop-mediated isothermal amplification (LAMP) assay using (Rv1980c) and (Rv0934) targets for the detection of in OATB patients. The sensitivities of 100 and 82.4% were obtained in confirmed (n = 10) and suspected (n = 57) OATB cases, respectively by multi-targeted LAMP with a specificity of 96.9% (n = 33). Moreover, the sensitivities attained by multi-targeted LAMP in total OATB cases were significantly higher (p < 0.05-0.01) than multiplex PCR ( + ) and GeneXpert assay. Our LAMP is simple, reliable and cost-effective method, which may develop into an attractive diagnostic kit for early detection of OATB cases.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2217/fmb-2021-0030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Musculoskeletal infection, diagnosis and management.
J Clin Orthop Trauma
March 2025
Southport & Ormskirk Hospitals, Mersey & West Lancashire Teaching NHS Trust, UK.
Unique Subclavian Vascular Ring Anomaly: Insights from CT Angiography.
Life (Basel)
January 2025
Clinical Laboratory of Radiology and Medical Imaging, "Sf. Apostol Andrei" County Emergency Hospital, 900591 Constanta, Romania.
Aortic arch anomalies represent a range of congenital vascular malformations resulting from disruptions in the typical embryological development of the aortic arch and its branches. These anomalies, which vary widely in their presentation, can lead to significant clinical symptoms depending on their structure and position. We report the case of a 75-year-old male with intermittent hypertension, palpitations, and episodic warmth in the upper body.
View Article and Find Full Text PDFTuberculous Osteomyelitis of the Scapular Spine Revealing HIV-1 Infection.
Trop Med Infect Dis
December 2024
Department of Infectious Diseases, University Hospital Mohamed VI, Marrakesh, Faculty of Medicine and Pharmacy FMPM, Cadi Ayyad University, Marrakesh 40000, Morocco.
Tuberculosis is no longer confined to developing nations; it persists as a significant contributor to illness and death on a global scale. The subtle clinical manifestation and association with human immunodeficiency virus infection poses obstacles for early diagnosis and management. Tuberculosis manifesting at extrapulmonary sites is relatively rare.
View Article and Find Full Text PDFThe Relevance of the Virchow Node and Virchow Triad in Renal Cancer Diagnosis.
Clin Pract
January 2025
Department of Cardiology and Internal Medicine, Colțea Clinical Hospital, 030167 Bucharest, Romania.
The purpose of this article is to overview the clinical significance of left supraclavicular adenopathy and review the etiology of inferior vena cava (IVC) thrombosis, starting from a presentation of a rare case of renal cell carcinoma (RCCs) with Xp11.2 translocation involving TFE3 gene fusion. This article also aims to review the literature to understand the characteristics of this rare type of renal tumor.
View Article and Find Full Text PDFMonocyte STAT1 phosphorylation and treatment response of JAK inhibitors in chronic nonbacterial osteomyelitis.
Pediatr Rheumatol Online J
January 2025
Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan.
Background: Chronic nonbacterial osteomyelitis (CNO) is a rare autoinflammatory disease of unknown cause, predominantly affecting teens and young adults. The early diagnosis and management are challenging due to the lack of reliable diagnostic markers and the occasional intractable cases despite conventional anti-inflammatory treatments. Janus kinase (JAK) inhibitors have recently shown potential utility; however, reports on their use for pediatric patients with CNO remain limited, and no established biomarkers exist to monitor disease activity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!