Restricted Access Molecularly Imprinted Polymers.

The use of conventional molecularly imprinted polymers (MIPs) for biological sample preparation is a difficult procedure due to the presence of high concentrations of proteins which can obstruct the selective binding sites, decrease the adsorption capacity, and compromise the analytical validation. In this way, modifications of conventional MIPs have been carried out in order to give them the ability to exclude macromolecules. Superficial coverings with hydrophilic groups and/or proteins have been the main procedures to obtain these restricted access molecularly imprinted polymers (RAMIPs ). These materials have been efficiently used for the selective extraction of small molecules from untreated complex matrices (e.g., blood, plasma, serum, and milk), without the need of a pre-deproteinization step. In this chapter, we describe a generic synthesis protocol to obtain RAMIPs as well as the assays to evaluate the protein exclusion efficiency and possible applications in offline and online procedures.