Molecularly Imprinted Polymeric Nanoparticles by Precipitation Polymerization and Characterization by Quantitative NMR Spectroscopy.

An optimized synthetic methodology for the preparation of highly homogeneous MIP nanoparticles by the precipitation method is presented. A quantitative H NMR method that was developed to estimate template incorporation, polymer composition and conversion, and binding capacities and selectivities is also described. While the experiment presented here is exemplified by an MIP formulation using (±)-propranolol as the template, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the crosslinker, the methods and techniques are applicable to other precipitation MIP systems.