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Monitoring Emergence of the SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). | LitMetric

AI Article Synopsis

  • - The B.1.1.7 variant of SARS-CoV-2, first found in the UK in late 2020, became prevalent in several countries, prompting concerns and research into tracking its spread.
  • - Researchers developed a specialized RT-qPCR assay to detect this variant and used wastewater samples in Spain to monitor its presence, revealing that the variant was identified earlier than reported clinical cases.
  • - Wastewater analysis showed a strong correlation with clinical data, indicating that B.1.1.7 could become dominant in about 8 weeks, suggesting that wastewater tracking is a cost-effective method for monitoring COVID-19 variants.

Article Abstract

Since its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, and used it to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than clinical epidemiological reporting by the local authorities, first in the southern city of Málaga (Andalucía) in week 20_52 (year_week), and multiple introductions during Christmas holidays were inferred in different parts of the country. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from wastewater treatment plants, which reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that 8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern as soon as they are identified by clinical sequencing and their integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404293PMC
http://dx.doi.org/10.1021/acs.est.1c03589DOI Listing

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