AI Article Synopsis
- - Virulence assays are important for understanding how microbes cause disease, particularly in plants, by monitoring disease progression and identifying factors that contribute to the pathogen's ability to cause illness.
- - Environmental conditions like temperature and humidity can greatly influence disease development, making it difficult to maintain consistent results in experiments.
- - This study outlines improved and validated methods for nine specific assays that evaluate virulence in a type of plant bacterium, using tomatoes to test these conditions.
Article Abstract
Virulence assays are powerful tools to study microbial pathogenesis . Good assays track disease development and, coupled with targeted mutagenesis, can identify pathogen virulence factors. Disease development in plants is extremely sensitive to environmental factors such as temperature, atmospheric humidity, and soil water level, so it can be challenging to standardize conditions to achieve consistent results. Here, we present optimized and validated experimental conditions and analysis methods for nine assays that measure specific aspects of virulence in the phytopathogenic bacterium , using tomato as the model host plant.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328803 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.3028 | DOI Listing |
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