Brome mosaic virus (BMV) is a well-known plant virus representing single-stranded RNA (ssRNA) positive-sense viruses. It has been widely used as a model in multiple studies concerning plant virus biology, epidemiology and the application of viral capsids in nanotechnology. Herein, we describe a method for BMV purification based on ion-exchange and size-exclusion chromatography. The presented method is of similar efficiency to previously described protocols relying on differential centrifugation and can easily be scaled up. The resulting BMV capsids are stable and monodisperse and can be used for further applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328587 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2935 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of an immunodiagnostic assay for the detection of .
Turk J Biol
August 2024
Department of Agricultural Sciences and Technology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.
Background/aim: (SCMV; genus and family ), poses a significant threat to global sugarcane cultivars, including those in Pakistan. The aim of this study was to develop a rapid and effective diagnostic tool for detection of SCMV, enabling timely implementation of control measures to mitigate potential yield losses.
Materials And Methods: The study focused on the in silico analysis, physicochemical properties, immunogenicity, and subcellular localization of the SCMV coat protein (CP).
Zymocin-like killer toxin gene clusters in the nuclear genomes of filamentous fungi.
Fungal Genet Biol
January 2025
Conway Institute and School of Medicine, University College Dublin, Dublin 4, Ireland. Electronic address:
Zymocin-like killer toxins are anticodon nucleases secreted by some budding yeast species, which kill competitor yeasts by cleaving tRNA molecules. They are encoded by virus-like elements (VLEs), cytosolic linear DNA molecules that are also called killer plasmids. To date, toxins of this type have been found only in budding yeast species (Saccharomycotina).
View Article and Find Full Text PDFTofacitinib Mitigates the Increased SARS-CoV-2 Infection Susceptibility Caused by an IBD Risk Variant in the PTPN2 Gene.
Cell Mol Gastroenterol Hepatol
January 2025
Division of Biomedical Sciences, School of Medicine, University of California Riverside, Riverside, California, USA. Electronic address:
Background: Coronavirus disease (COVID-19), caused by SARS-CoV-2, triggered a global pandemic with severe medical and socioeconomic consequences. While fatality rates are higher among the elderly and those with underlying comorbidities, host factors that promote susceptibility to SARS-CoV-2 infection and severe disease are poorly understood. Although individuals with certain autoimmune/inflammatory disorders show increased susceptibility to viral infections, there is incomplete knowledge of SARS-CoV-2 susceptibility in these diseases.
View Article and Find Full Text PDFFull-length genome reveals genetic diversity and extensive recombination patterns of Saudi GI-1 and GI-23 genotypes of infectious bronchitis virus.
Virol J
January 2025
Department of Microbiology, College of Medicine, Taif University, Taif, 21944, Saudi Arabia.
Background: Despite numerous genetic studies on Infectious Bronchitis Virus (IBV), many strains from the Middle East remain misclassified or unclassified. Genotype 1 (GI-1) is found globally, while genotype 23 (GI-23) has emerged as the predominant genotype in the Middle East region, evolving continuously through inter- and intra-genotypic recombination. The GI-23 genotype is now enzootic in Europe and Asia.
View Article and Find Full Text PDFRsWOX13 promotes taproot development by activating cell division and expansion and sucrose metabolism in radish.
Plant Physiol Biochem
December 2024
National Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China) of MOAR, College of Horticulture, Sanya Institute, Nanjing Agricultural University, Zhongshan Biological Breeding Laboratory, Nanjing, 210095, PR China; College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, 225009, China. Electronic address:
Radish is an important annual root vegetable crop, whose yield is largely dependent on taproot thickening and development. However, the regulatory network of WOXs-mediated taproot development remains poorly understood in radish. Herein, the RsWOX13 was classified in an ancient clade of the WOX gene family that harbors a conserved homeodomain.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!