Comparing Bacterial Leakage of Three Intraorifice Barrier Sealing Materials against and .

Aim: The purpose of this study was to evaluate the intraorifice sealing ability of light-cured glass-ionomer cement (LC-GIC), Tetric N-Flow, and ProRoot mineral trioxide aggregate (MTA) against and .

Materials And Methods: Crowns of the eighty human mandibular teeth were decapitated. Working length determination was performed, after which cleaning and shaping were carried out. A uniform orifice diameter of 1.3 mm, at its widest point, was made. Once instrumentation was completed, the canals were irrigated and then obturated. A heat carrier was used to remove gutta-percha to the depth of 3.5 mm. Samples were then divided into a control group (Group 1) with no barrier, and three groups, namely, Group 2, Group 3, and Group 4, were restored with the LC-GIC, Tetric N-Flow, and ProRoot MTA, respectively. The groups were further subdivided into Subgroup A for checking bacterial leakage against and Subgroup B, against . All samples were subjected to the bacterial leakage test and observed daily for the appearance of turbidity after which statistical analysis was performed.

Results: Group 1 showed leakage in, as early as, 3 days. The longest time for the turbidity to appear was shown by Group 4 with an average of 31 days. The mean number of days for turbidity to appear in Group 2 and Group 3 was 23 and 24 days, respectively. Group 4 showed the best intraorifice sealing ability with a significant difference.

Conclusion: The teeth with an intraorifice coronal seal had better protection against microbial leakage. Among all materials used, the ProRoot MTA showed the best intraorifice sealing ability.

Clinical Significance: Use of the ProRoot MTA promises long-term results in the endodontically treated teeth as compared with other materials.