In vivo and in vitro antiproliferative and antimetastatic effects of hemolymph of Aspongopus chinensis Dallas on breast cancer cell.

Objective: To investigate the effects of Aspongopus（A.）chinensis hemolymph on the proliferation and metastasis of breast cancer cells.

Methods: The in vitro effects of A. chinensis hemolymph were investigated in murine (4T1) and human (HCC1937) breast cancer cell lines. Cytotoxicity, cell apoptosis, and cell migration were evaluated by using the cell counting kit-8 assay, Hoechst staining, and wound healing experiments, respectively. A syngeneic mouse model was established to evaluate the in vivo effects of the hemolymph extract on tumor growth and metastasis. Mouse body weight, tumor size, blood levels of function-related enzymes, and pathological features of the liver and kidney tissues were evaluated.

Results: The hemolymph of A. chinensis significantly inhibited in vitro tumor cell migration and viability while inducing apoptosis. Furthermore, it inhibited in vivo tumor growth and metastasis with a minimal effect on mouse body weight, and did not induce liver or kidney damage.

Conclusion: Our results suggested that the A.chinensis hemolymph has antitumorigenic properties, suggesting it has potential as a novel therapeutic option for the treatment of metastatic breast cancer.