Characterization, Identification and Evaluation of Wheat- Chromosome Derivatives.

, a wild relative of wheat, harbors diverse disease and insect resistance genes, making it a potentially excellent gene source for wheat improvement. In this study, we characterized and evaluated six wheat- derivatives, which included three disomic additions, one disomic substitution + monotelosomic addition and two disomic substitution + disomic additions. A total of 51 PLUG markers were developed and used to allocate the chromosomes in each of the six derivatives to homoeologous groups. A set of cytogenetic markers specific for chromosomes was established based on FISH using oligonucleotides as probes. Molecular cytogenetic marker analysis confirmed that these lines were a CS-. 2S disomic addition, a 4S disomic addition, a 4S (4D) substitution + 5SL monotelosomic addition, a 6S disomic addition, a 4S (4D) substitution + 6S disomic addition and a 4S (4D) substitution + 7S disomic addition line, respectively. Disease resistance investigations showed that chromosome 7S of . might harbor a new powdery mildew resistance gene, and therefore it has potential for use as resistance source for wheat breeding.