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This study describes JE7A12 (=ATCC TSD-225=NCTC 14479), an isolate from the ruminal content of a dairy cow. Phenotypic and genotypic traits of the isolate were explored. JE7A12 was found to be a strictly anaerobic, catalase-negative, oxidase-negative, coccoid bacterium that grows in chains. The API 50 CH carbon source assay detected fermentation of d-glucose, d-fructose, d-galactose, glycogen and starch. HPLC showed acetate to be the major fermentation product as a result of carbohydrate fermentation. Phylogenetic analysis of JE7A12 based on 16S rRNA nucleotide sequence and amino acid sequences from the whole genome indicated a divergent lineage from the closest neighbours in the genus . The results of 16S rRNA sequence comparison, whole genome average nucleotide identity (ANI) and DNA G+C content data indicate that JE7A12 represents a novel species which we propose the name with JE7A12 as the type strain.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513621 | PMC |
| http://dx.doi.org/10.1099/ijsem.0.004924 | DOI Listing |
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