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sp. nov., isolated from larvae of , proposal of gen. nov., comb. nov. and emended descriptions of the genera , and . | LitMetric
Taxonomic positions of four Gram-negative bacterial strains, which were isolated from larvae of two insects in Jeju, Republic of Korea, were determined by a polyphasic approach. Strains CWB-B4, CWB-B41 and CWB-B43 were recovered from larvae of , whereas strain BWR-B9 was from larvae of . All the isolates grew at 10-37 °C, at pH 5.0-9.0 and in the presence of 4 % (w/v) NaCl. The 16S rRNA gene phylogeny showed that the four isolates formed two distinct sublines within the order and closely associated with members of the genus . The first group represented by strain CWB-B4 formed a tight cluster with CF-1111 (99.3 % sequence similarity), whereas strain BWR-B9 was most closely related to CF-458 (99.5 % sequence similarity). The 92 core gene analysis showed that the isolates belonged to the family and supported the clustering shown in 16S rRNA gene phylogeny. The genomic DNA G+C content of the isolates was 45.2 mol%. A combination of overall genomic relatedness and phenotypic distinctness supported that three isolates from are different strains of , whereas one isolate from represents a new species of the genus . On the basis of results obtained here, sp. nov. (type strain BWR-B9=KACC 22153=NBRC 114879) and gen. nov., comb. nov. are proposed, with the emended descriptions of the genera , and .
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