AI Article Synopsis
- Neuraminidase (NA) is a key protein on influenza A viruses, and specific NA1-based virus-like particles (VLPs) have shown protective effects against certain strains.
- Researchers produced NA2 VLPs from the A/Perth/16/2009 strain, which induced a strong immune response in mice, providing protection against lethal H3N2 virus but not H1N1.
- Combination vaccination with both NA1 and NA2 VLPs resulted in effective protection against both H1N1 and H3N2 viruses, highlighting the potential for focusing on anti-NA responses in future flu vaccine development.
Article Abstract
Neuraminidase (NA) is the second most abundant glycoprotein on the surface of influenza A viruses (IAV). Neuraminidase type 1 (NA1) based virus-like particles (VLPs) have previously been shown to protect against challenge with H1N1 and H3N2 IAV. In this study, we produced neuraminidase type 2 (NA2) VLPs derived from the sequence of the seasonal IAV A/Perth/16/2009. Intramuscular vaccination of mice with NA2 VLPs induced high anti-NA serum IgG levels capable of inhibiting NA activity. NA2 VLP vaccination protected against mortality in a lethal A/Hong Kong/1/1968 (H3N2) virus challenge model, but not against lethal challenge with A/California/04/2009 (H1N1) virus. However, bivalent vaccination with NA1 and NA2 VLPs demonstrated no antigenic competition in anti-NA IgG responses and protected against lethal challenge with H1N1 and H3N2 viruses. Here we demonstrate that vaccination with NA VLPs is protective against influenza challenge and supports focusing on anti-NA responses in the development of future vaccination strategies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479372 | PMC |
| http://dx.doi.org/10.1016/j.virol.2021.08.001 | DOI Listing |
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