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The effect of cisplatin-low-level laser therapy on cell viability and death of LNCaP prostate cancer cell line. | LitMetric

AI Article Synopsis

  • - Prostate cancer is a major health issue for men, and despite advancements in treatment, options like surgery, radiation, and chemotherapy have limitations that impact survival.
  • - This study explored the effects of combining cisplatin, a chemotherapy drug, with low-level laser treatment (LLLT) on the LNCaP prostate cancer cell line to see if it improved treatment outcomes.
  • - Results indicated that this combination therapy effectively reduced cell viability and cytotoxicity while increasing lipid peroxidation and LDH enzyme activity, suggesting it could be a promising approach for better cancer treatment with fewer side effects.

Article Abstract

Prostate cancer, as a common male cancer, is a serious threat to men's health. In spite of extreme developments for increasing survival rate, there are still limitations about common treatment options such as surgical procedures, radiotherapy, and chemotherapy. We hypothesized that combination of two treatments would bring better clinical outcomes. Therefore, the aim of this study was to determine the effect of conjugated cisplatin and low-level laser treatment (LLLT) on the viability of LNCaP prostate cancer cell line. LNCaP cells were harvested in DMEM containing 10% FBS and 1% antibiotic. Confluent cells were treated with different concentrations of cisplatin and different wavelengths of low-level laser (LLL) alone and in combination. The relative IC50 and cell viability was evaluated using MTT assay. Analysis of lipid peroxidation rate was performed using lipid peroxidation assay kit. LDH activity was also carried out on the treated and control cells using LDH cytotoxicity assay kit. Our results showed that combination of cisplatin and LLLT could effectively decrease cisplatin-induced cytotoxicity as well as LNCaP cell viability. Cisplatin-LLLT combination led to a significant increase in the MDA content as the product of membrane lipid peroxidation. Analyzing the LDH activity under the effect of cisplatin-LLL combined treatment showed a remarkable increase in the enzyme activity. We conclude that applying the cisplatin-LLL combination therapy is promising as an effective anti-cancer treatment. This novel combination has a potential to attenuate adverse side effects of earlier monotherapy strategies.

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Source
http://dx.doi.org/10.1007/s10103-021-03386-yDOI Listing

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