Trypan blue staining is a classic way of visualizing leaf disease and wound responses in plants, but it involves working with toxic chemicals and is time-consuming (2-3 days). Here, the investigators established near-infrared scanning with standard lab equipment as a fast and nondestructive method for the analysis of leaf injuries compared with trypan blue staining. Pathogen-inoculated and wounded leaves from potato, tomato, spinach, strawberry, and arabidopsis plants were used for proof of concept. The results showed that this newly developed protocol with near-infrared scanning gave the same results as trypan blue staining. Furthermore, a macro in FIJI was made to quantify the leaf damage. The new protocol was time-efficient, nondestructive, chemical-free and may be used for high-throughput studies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2144/btn-2021-0045 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluating toxicity and level of DNA damage in human fetal lung cells upon exposure to 5-methyltetrahydrofolate (bioactive folate).
Nutr Health
December 2024
Biology and Environmental Science Department, University of New Haven, West Haven, CT, USA.
Folic acid (FA) supplementation is widely regarded as a key nutritional intervention during pregnancy due to its protective effect against neural tube defects. Recent research has reported FA supplementation outcomes on offspring's health, with increased incidences of allergy/respiratory problems. This study evaluates if increased levels of 5-methyltetrahydrofolate (5-MTHF) are associated with DNA modification, leading to disruption of cell proliferation in fetal lung cells and increasing susceptibility to asthma.
View Article and Find Full Text PDFScreening Algal and Cyanobacterial Extracts to Identify Potential Substitutes for Fetal Bovine Serum in Cellular Meat Cultivation.
Foods
November 2024
Department of Biochemistry & Center of Excellence for Molecular Food Sciences, University of Belgrade-Faculty of Chemistry, 11000 Belgrade, Serbia.
Cultured meat technology is a form of cellular agriculture where meat is produced from animal cells grown in a lab, instead of raising and slaughtering animals. This technology relies heavily on fetal bovine serum (FBS) in cell media; hence, production is costly and contributes significantly to ammonia and greenhouse gas emissions. Achieving the successful commercialization of cell-cultured food requires the critical resolution of manufacturing cost and safety concerns.
View Article and Find Full Text PDFAntibiofilm Analysis, Synergistic Potential and Biocompatibility Evaluation of a Bacteriocin from (MK733983).
Indian J Microbiol
December 2024
Department of Chemistry & Biochemistry, Jain (Deemed to-Be) University, 34, JC Road, Bengaluru, Karnataka 560027 India.
This study emphasizes the potency of a bacteriocin screened from (MK733983) of ethnomedicinal origin. Antibiofilm analysis with 0.5-3x minimal bacteriocin concentrations with critical and highly prioritized standard microbes such as , , , , , and showed potential biofilm inhibition and eradication of ≥ 5-99%, ≥ 1-86% respectively that correlated with biofilm viable cell-count.
View Article and Find Full Text PDFSynthesis and pharmacological evaluation of heteroarylamide derivatives as potential analgesic, anti-inflammatory, antidiarrheal and cytotoxic agents.
Heliyon
December 2024
Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka, 1000, Bangladesh.
Six heteroarylamide derivatives were synthesized in good yields and screened for several biological activities. Compounds demonstrated analgesic activity with percentage inhibition of writhing between 77.10 and 95.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!