Patients with diabetes frequently suffer from periodontitis, which progresses rapidly and is difficult to cure. Mesenchymal stem cell (MSC) transplantation may effectively treat periodontitis, but high glucose limits its therapeutic effect in diabetes. Nerve growth factor (NGF) has the functions of cell protection, anti-apoptosis and immune regulation, and may have potential application in diabetic periodontitis. In the present study, flow cytometry indicated that NGF inhibited MSC apoptosis induced by high glucose. Of note, high glucose promoted the transformation of MSCs into the proinflammatory type. NGF inhibited this transformation of MSCs under diabetic conditions and further decreased the proportion of T cells and monocytes/macrophages among lymphocytes. An animal model of diabetic periodontitis was constructed and MSC transplantation was demonstrated to reduce alveolar bone loss caused by diabetes. NGF enhanced the therapeutic effect of MSCs and maintained transplanted MSC survival in periodontal tissue of diabetic mice. Immunohistochemical analysis of periodontal tissues suggested that in the NGF group, infiltration of T cells and macrophages was reduced. Neurotrophic receptor tyrosine kinase 1 was indicated to have a key role in these effects of NGF. In conclusion, NGF may enhance the therapeutic effect of MSCs on diabetic periodontitis by protecting the cells and promoting the transformation of MSCs into the immunosuppressive type.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343805 | PMC |
| http://dx.doi.org/10.3892/etm.2021.10445 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Periodontal pathogens and obesity in the context of cardiovascular risks across age groups.
Front Oral Health
January 2025
Department of Cardiovascular Pathology and Diet Therapy, Federal Research Centre for Nutrition, Biotechnology and Food Safety, Moscow, Russia.
Background: Cardiovascular diseases (CVDs) are the leading cause of mortality and morbidity among noncommunicable diseases. Over the past decade, there has been a notable increase in the prevalence of CVDs among young individuals. Obesity, a well-known risk factor for CVDs, is also associated with various comorbidities that may contribute to cardiovascular risk.
View Article and Find Full Text PDFSalivary proteomic analysis in patients with type 2 diabetes mellitus and periodontitis.
Clin Oral Investig
January 2025
Institute of Science and Technology, Division of Periodontics, São Paulo State University (Unesp), Av. Eng. Francisco José Longo, 777, São José dos Campos, São Paulo, 12245-000, Brazil.
Objective: This study aimed to compare the salivary protein profile in individuals with Type 2 Diabetes Mellitus (DM2) and periodontitis and their respective controls.
Methods: Eighty participants were included in the study. The four groups were formed by individuals with DM2 and periodontitis (DM2 + P, n = 20), DM2 without periodontitis (DM2, n = 20), periodontitis without DM2 (P, n = 20) and individuals without periodontitis and without DM2 (H, n = 20).
Effect of ozone oil and non-surgical periodontal treatment in patients with type 2 diabetes. In-vivo and in-vitro studies with fibroblasts and Candida albicans.
J Appl Oral Sci
January 2025
Universidade Federal Fluminense, Instituto de Saúde de Nova Friburgo, Departamento de Clínica Odontológica, Nova Friburgo, Rio de Janeiro, Brasil.
Aim: To evaluate the clinical effectiveness of ozonated sunflower oil (Oz) as an adjunctive of non-surgical periodontal therapy in patients with type 2 diabetes mellitus (DM2), on fibroblast cell viability and migration and the effectiveness of Oz on a Candida albicans (C. albicans) culture.
Methodology: In total, 32 sites in 16 DM2 with moderate to advanced periodontal disease with periodontal pocket depths ≥5mm were selected.
Conventional periodontal probing versus salivary biomarkers in diagnosis and evaluation of chronic periodontitis in type 2 diabetes mellitus.
Minerva Dent Oral Sci
January 2025
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, India -
Chronic periodontitis is a prevalent inflammatory condition that affects both the oral health and systemic well-being of individuals, particularly those with type 2 diabetes mellitus (T2DM). Accurate diagnosis and evaluation of periodontal status are crucial for effective management of the disease. This narrative review aimed to compare the diagnostic and evaluative capabilities of conventional periodontal probing and salivary biomarkers in chronic periodontitis among individuals with T2DM.
View Article and Find Full Text PDFMicrofluidic organ-on-chip systems for periodontal research: advances and future directions.
Front Bioeng Biotechnol
January 2025
Faculty of Dentistry, National University of Singapore, Singapore, Singapore.
Advances in tissue engineering and microfluidic technologies have enabled the development of sophisticated models known as organ-on-a-chip (OoC) or microphysiological systems. These systems enable to potential to simulate the dynamic interactions between host tissues and their microenvironment including microbes, biomaterials, mechanical forces, pharmaceutical, and consumer-care products. These fluidic technologies are increasingly being utilized to investigate host-microbe and host-material interactions in oral health and disease.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!