Severity: Warning
Message: fopen(/var/lib/php/sessions/ci_sessionhr6eg8eqtsshkt3rbr7gjrcjoeti1bk6): Failed to open stream: No space left on device
Filename: drivers/Session_files_driver.php
Line Number: 177
Backtrace:
File: /var/www/html/index.php
Line: 316
Function: require_once
Severity: Warning
Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)
Filename: Session/Session.php
Line Number: 137
Backtrace:
File: /var/www/html/index.php
Line: 316
Function: require_once
Background: β-Tricalcium phosphate (β-TCP) is a popular synthetic bone graft substitute with excellent osteoconductive properties and bioabsorbability. However, its osteoinductive properties are inferior to those of autologous or allogeneic bone. Trace elements such as strontium (Sr), silica (Si), and zinc (Zn) have been reported to promote osteogenesis in materials. In this study, we aimed to determine whether a Si/Zn-substituted Sr apatite coating of β-TCP could enhance osteoinductive properties.
Methods: The apatite-coated β-TCP disks were prepared using nanoparticle suspensions of silicate-substituted Sr apatite (SrSiP) or silicate- and Zn-co-substituted Sr apatite (SrZnSiP). Bone marrow mesenchymal cells (BMSCs) from rat femur were cultured and subsequently seeded at a density of 1.0 × 10/cm onto apatite-coated and non-coated β-TCP disks. In vitro, the β-TCP disks were then placed in osteogenic medium, and lactate dehydrogenase (LDH) activity was measured from supernatants after culture for 2 days. Additionally, after culture for 14 days, the mRNA expression of genes encoding osteocalcin (OC), alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP-2), and vascular endothelial growth factor (VEGF) was evaluated by qRT-PCR. In vivo, the β-TCP disks were transplanted subcutaneously into rats that were sacrificed after 4 weeks. Then, the harvested disks were evaluated biochemically (ALP activity, OC content, mRNA expression of OC, ALP, BMP-2, and VEGF measured by qRT-PCR), radiologically, and histologically.
Results: Significantly higher mRNA expression of almost all evaluated osteogenic and angiogenic genes was observed in the SrZnSiP and SrSiP groups than in the non-coated group, with no significant cytotoxicity elicited by the apatite coating in vitro. Moreover, in vivo, the SrZnSiP and SrSiP groups showed significantly higher osteogenic and angiogenic gene expression and higher ALP activity and OC content than the non-coated group (P < 0.05). Radiological and histopathological findings revealed abundant bone formation in the apatite-coated group.
Conclusions: Our findings indicate that apatite coating of β-TCP improves osteoinductive properties without inducing significant cytotoxicity.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353809 | PMC |
http://dx.doi.org/10.1186/s12891-021-04563-4 | DOI Listing |
Objective: To evaluate color masking and relative translucency parameter (RTP) of increasing dentin thicknesses from different resin composites, with or without opacifiers, on a veneer dental preparation and resin disks.
Material And Methods: Artificial darkened lateral incisors with 1mm-thick veneers preparations were used to evaluate color masking of different resinous materials, with or without opacifiers: IPS Empress Direct (ED) with or without ED Opaque; and Essentia (ES) with or without ES Masking Liner. For the RTP test; disc-shaped specimens were performed and evaluated with a spectrophotometer (VITA Easyshade) against black and C4 backgrounds.
Chronic lower back pain is the leading cause of disability worldwide, generating a socioeconomic cost of over $100 billion annually in the United States. Among the prominent causes of low back pain (LBP) is degeneration of the intervertebral disk (IVD), a condition known as degenerative disk disease (DDD). Despite the prevalence of DDD and multiple studies demonstrating its relationship with LBP, the mechanisms by which it contributes to pain remain unknown.
View Article and Find Full Text PDFDrug Deliv Transl Res
December 2024
Department of Bioengineering, Rice University, 6500 Main Street, Houston, TX, 77030, USA.
Poly(glycerol sebacate) (PGS) is a biodegradable, elastomeric polymer that has been explored for applications including tissue engineering, drug delivery, and wound repair. Despite its promise, its biomedical utility is limited by its rapid, and largely fixed, degradation rate. Additionally, its preparation requires prolonged curing at high temperatures, rendering it incompatible with heat-sensitive molecules, complex device geometries, and high-throughput production.
View Article and Find Full Text PDFBMC Oral Health
December 2024
Department of Restorative Dentistry, Faculty of Dentistry, Gazi University, Ankara, Turkey.
Background: The purpose of this study was to compare AH Plus Bioceramic Sealer (AHPB), AH Plus (AHP), MTA Fillapex (MTAF), and Sure-Seal (SS) sealers for dentinal tubular penetration ability.
Methods: Forty extracted human mandibular premolars were instrumented and divided into 4 groups based on the sealer used for filling (n = 10): AHPB, AHP, MTAF, SS. After filling the root canals, the roots were entombed in acrylic resin blocks and cut vertically in the long axis to obtain 1-mm-thick disks from 3 mm (at apical third), 8 mm (at middle third) and 13 mm (at coronal third) away the apical foramen.
Int J Biol Macromol
December 2024
'Materials+Technology' Group, Engineering School of Gipuzkoa, Department of Chemical and Environmental Engineering, University of the Basque Country (UPV/EHU), Pza. Europa 1, 20018, Donostia-San Sebastián, Spain. Electronic address:
Thiolated chitosan (Cs-SH) nanoparticles were synthesized and incorporated into bacterial cellulose (BC) membranes through vacuum-assisted confinement. Thiolation significantly enhanced the intrinsic adhesion capacity of chitosan (Cs) as well as its solubility in neutral aqueous solutions. Subsequently, Cs-SH nanoparticles were successfully loaded with curcumin (Cur-Cs-SH), with nanoparticle sizes of 121 ± 2 nm for Cs-SH and 152 ± 6 nm for Cur-Cs-SH.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!