AI Article Synopsis
- - The advancements in next-generation sequencing technology and bioinformatics have made it easier and cheaper to assemble DNA sequences, especially for shorter organelle genomes like chloroplasts.
- - The increasing availability of complete chloroplast genome sequences in public databases is important for research in plant phylogenetics and biotechnology.
- - This study focused on identifying and correcting inconsistencies in publicly available chloroplast genome data, demonstrating the impacts of these inconsistencies on phylogenetic analysis using five different plant families as examples.
Article Abstract
With the development of next-generation sequencing technology and bioinformatics tools, the process of assembling DNA sequences has become cheaper and easier, especially in the case of much shorter organelle genomes. The number of available DNA sequences of complete chloroplast genomes in public genetic databases is constantly increasing and the data are widely used in plant phylogenetic and biotechnological research. In this work, we investigated possible inconsistencies in the stored form of publicly available chloroplast genome sequence data. The impact of these inconsistencies on the results of the phylogenetic analysis was investigated and the bioinformatic solution to identify and correct inconsistencies was implemented. The whole procedure was demonstrated using five plant families (Apiaceae, Asteraceae, Campanulaceae, Lamiaceae and Rosaceae) as examples.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309291 | PMC |
| http://dx.doi.org/10.3390/plants10071360 | DOI Listing |
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