AI Article Synopsis

  • The study focuses on the MAPK gene (RmMAPK) from the cold-active yeast Rhodotorula mucilaginosa and its role in stress responses.
  • The RmMAPK gene, which is 1086 bp long, encodes a protein that plays a crucial role in signaling during extreme conditions, showing significant expression and phosphorylation under stressors like low temperatures, high salinity, and heavy metals.
  • Findings indicate that MAPK pathways are vital for the yeast’s adaptation to harsh environments.

Article Abstract

Background: The mitogen-activated protein kinase (MAPK) cascades play important roles in various signaling transduction networks of biotic and abiotic stress responses. However, MAPK signaling pathways in cold-active yeast Rhodotorula mucilaginosa have not been reported comprehensively.

Methods And Results: In the present study, MAPK gene (RmMAPK) was first cloned and characterized from Antarctic sea ice yeast R. mucilaginosa AN5. The full length of the RmMAPK gene is 1086 bp and encodes a 361 amino acids protein with a predicted molecular mass of 40.9 kDa and a pI of 5.25. The RmMAPK contains 11 MAPK conserved subdomains and the phosphorylation motif TGY located in the activation loop of the kinase. Quantitative real-time PCR and western blot assay revealed that the expression and phosphorylation level of RmMAPK up-regulated rapidly and significantly when yeast cells were subjected to low temperature (4 °C), high salinity (120‰ NaCl) and heavy metal (2 mmol/L CuCl).

Conclusions: All data suggested that the MAPK cascades might act as a key function in response to extreme stresses, such as low temperature, high salinity and heavy metal.

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Source
http://dx.doi.org/10.1007/s11033-021-06570-zDOI Listing

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