AI Article Synopsis
- KLF4 and KLF5 are transcription factors linked to gastrointestinal tumors, and their roles were examined using bioinformatics analysis of various databases.
- Data analysis methods included gene expression profiling, survival analysis, and enrichment studies, along with laboratory assays to test KLF5's effects on gastric cancer cells.
- Findings indicate that KLF4 and KLF5 are differentially expressed in tumor versus normal tissues, with KLF5 promoting cancer cell growth and contributing to metastasis, highlighting their potential as therapeutic targets in gastrointestinal cancer treatment.
Article Abstract
Background: KLF4 and KLF5 are members of the KLF transcription factor family, which play an important role in many gastrointestinal tumors. To gain a deeper insight into its function and role, bioinformatics was used to analyze the function and role of KLF4 and KLF5 in gastrointestinal tumors.
Methods: Data were collected from several online databases. Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN database analysis, Kaplan-Meier Plotter analysis, LOGpc system, the Pathology Atlas, and the STRING website were used to analyze the data. We download relevant data from TCGA and then perform GO enrichment and KEGG enrichment analysis. The effects of KLF5 on gastric cancer cell proliferation were measured by CCK-8 assay. The effect of KLF5 on the expression of CyclinD1 and MMP9 was detected by Western blot.
Results: KLF4 and KLF5 were differentially expressed in normal and tumor tissues of the gastrointestinal tract, and their differential expression is related to several genes or pathways. KEGG analysis showed that KLF5 was coexpressed with endocytosis-related genes. KLF5 promotes the proliferation of gastric cancer cells and the expression of metastasis-related molecules.
Conclusion: KLF4 and KLF5 are of great significance for developing gastrointestinal tumors and can be used as therapeutic targets.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8337138 | PMC |
| http://dx.doi.org/10.1155/2021/2425356 | DOI Listing |
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