AI Article Synopsis
- DNA damage is harmful and linked to aging, cancer, and diseases, prompting cells to develop effective DNA repair mechanisms.
- Traditional DNA repair assays are often low throughput and only examine a single protein or repair pathway, limiting their effectiveness.
- The CometChip platform enhances the traditional comet assay by enabling simultaneous analysis of multiple DNA repair activities, proving effective for different repair pathways and increasing overall sensitivity and throughput.
Article Abstract
DNA damage can be cytotoxic and mutagenic, and it is directly linked to aging, cancer, and other diseases. To counteract the deleterious effects of DNA damage, cells have evolved highly conserved DNA repair pathways. Many commonly used DNA repair assays are relatively low throughput and are limited to analysis of one protein or one pathway. Here, we have explored the capacity of the CometChip platform for parallel analysis of multiple DNA repair activities. Taking advantage of the versatility of the traditional comet assay and leveraging micropatterning techniques, the CometChip platform offers increased throughput and sensitivity compared to the traditional comet assay. By exposing cells to DNA damaging agents that create substrates of Base Excision Repair, Nucleotide Excision Repair, and Non-Homologous End Joining, we show that the CometChip is an effective method for assessing repair deficiencies in all three pathways. With these applications of the CometChip platform, we expand the utility of the comet assay for precise, high-throughput, parallel analysis of multiple DNA repair activities.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439179 | PMC |
| http://dx.doi.org/10.1016/j.dnarep.2021.103176 | DOI Listing |
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