AI Article Synopsis
- mAm (N,2'-O-dimethyladenosine) is a recently identified reversible RNA modification found near the mRNA cap, but a precise method to map it across the transcriptome was previously unavailable.
- mAm-seq is a new technique developed to specifically distinguish and identify mAm and 5'-UTR N-methyladenosine (mA) in the human transcriptome, achieving detailed mapping at single-base resolution.
- The study reveals that both mAm and 5'-UTR mA can change in response to various stimuli, highlighting important methylation sites involved in cellular stress responses and providing a valuable tool for further research into these RNA modifications.
Article Abstract
N,2'-O-dimethyladenosine (mAm), a terminal modification adjacent to the mRNA cap, is a newly discovered reversible RNA modification. Yet, a specific and sensitive tool to directly map transcriptome-wide mAm is lacking. Here, we report mAm-seq, based on selective in vitro demethylation and RNA immunoprecipitation. mAm-seq directly distinguishes mAm and 5'-UTR N-methyladenosine (mA) and enables the identification of mAm at single-base resolution and 5'-UTR mA in the human transcriptome. Using mAm-seq, we also find that mAm and 5'-UTR mA respond dynamically to stimuli, and identify key functional methylation sites that may facilitate cellular stress response. Collectively, mAm-seq reveals the high-confidence mAm and 5'-UTR mA methylome and provides a robust tool for functional studies of the two epitranscriptomic marks.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346571 | PMC |
| http://dx.doi.org/10.1038/s41467-021-25105-5 | DOI Listing |
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