The 1H-n.m.r. spectra (200 MHz) of protonated (pD 3.9) and non-protonated (pD 11.9) tobramycin have been analysed completely by 2D methods. The 3JH,H values are consistent with an essentially undistorted 4C1 conformation for each of the three moieties and are practically independent of the state of protonation. The resonances in the 13C-n.m.r. spectrum (50 MHz) have been reassigned at both pD values on the basis of 2D1H-13C chemical-shift correlation and 1D selective INEPT measurements.
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