It was previously demonstrated that predatory bacteria are able to efficiently eliminate Gram-negative pathogens including antibiotic-resistant and biofilm-associated bacteria. In this proof-of-concept study we evaluated whether two species of predatory bacteria, and , were able to alter the survival of Gram-negative pathogens on the ocular surface. Clinical keratitis isolates of (strain PAC) and (strain K904) were applied to the ocular surface of NZW rabbits followed by application of predatory bacteria. At time intervals, surviving pathogenic bacteria were enumerated. In addition, and were applied to porcine organ culture corneas under contact lenses, and the ocular surface was examined by scanning electron microscopy. The ocular surface epithelial layer of porcine corneas exposed to , but not was damaged. Using this model, neither pathogen could survive on the rabbit ocular surface for longer than 24 h. correlated with a more rapid clearance of but not from rabbit eyes. This study supports previous evidence that predatory bacteria are well tolerated by the cornea, but suggest that predatory bacteria do not considerably change the ability of the ocular surface to clear the tested Gram-negative bacterial pathogens from the ocular surface.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300752 | PMC |
| http://dx.doi.org/10.3390/antibiotics10070810 | DOI Listing |
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