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Excessive copper induces lignin biosynthesis in the leaves and roots of two citrus species: Physiological, metabolomic and anatomical aspects.

Ecotoxicol Environ Saf

January 2025

College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Lab of Soil Ecosystem Health and Regulation, Fujian Province University (Fujian Agriculture and Forestry University), Fuzhou 350002, China. Electronic address:

Excessive copper (Cu) of rhizosphere inhibited the growth and development of citrus seedlings. Lignin deposition on the cell wall promotes plant Cu tolerance. However, the lignin biosynthesis in citrus leaves and roots that respond to Cu toxicity is not fully understood.

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Morphological, Anatomical, and Histochemical Study of Cordia diffusa K.C. Jacob-A Steno Endemic Plant.

Microsc Res Tech

December 2024

Department of Botany, Root and Soil Biology Laboratory, Bharathiar University, Coimbatore, Tamil Nadu, India.

Cordia diffusa K.C. Jacob, known as Sirunaruvili, belonging to the family Boraginaceae, is a rare endemic species.

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Plant anatomical and histochemical studies are concerned with the structural organization of tissues as well as localization of various metabolites and enzyme activity inside cells and tissues. Traditionally, rotary microtomes are used for paraffin and resin-embedded samples which provide excellent preservation of tissue morphology but removes enzymes, lipid components, and various specialized metabolites. Freeze sectioning apparently remained unexplored in plant histology because of the presence of rigid cell walls and highly vacuolated cytoplasm in plant tissues.

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Understanding patterns of cortico-cortical connections in both frequently and infrequently studied species advances our knowledge of cortical organization and evolution. The agouti (Dasyprocta aguti, a medium-size South American rodent) offers a unique opportunity, because of its large lissencephalic brain and its natural behaviors, such as gnawing and hiding seeds, that require bimanual interaction while sitting on its hindlimbs and aligning its head to receive images of the horizon on the retinal visual streak. There have been no previous studies of the intrinsic and extrinsic ipsilateral projections of the agouti's primary somatosensory cortical area (S1).

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Article Synopsis
  • * Studies on Symplocos tetrandra using light and scanning electron microscopy revealed key anatomical features, such as calcium oxalate crystals and specialized stomata, useful for species identification.
  • * The presence of these metabolites and unique anatomical markers indicates potential for further pharmacological research and taxonomic classification of S. tetrandra.
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