Four marine bacterial strains were isolated from a thallus of the brown alga collected in Roscoff, France. Cells were Gram-stain-negative, strictly aerobic, non-flagellated, gliding, rod-shaped and grew optimally at 25-30 °C, at pH 7-8 and with 2-4 % NaCl. Phylogenetic analyses of their 16S rRNA gene sequences showed that the bacteria were affiliated to the genus (family , phylum ). The four strains exhibited 97.8-100 % 16S rRNA gene sequence similarity values among themselves, 97.9-99.1 % to the type strains of KMM 3526 and KMM 3676, and less than 99 % to other species of the genus . The DNA G+C content of the four strains ranged from 36.7 to 37.7 mol%. Average nucleotide identity and digital DNA-DNA hybridization calculations between the new strains and other members of the genus resulted in values of 76.4-88.9 % and below 38.5 %, respectively. Phenotypic, phylogenetic and genomic analyses showed that the four strains are distinct from species of the genus with validly published names. They represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed with Asnod1-F08 (RCC6906=KMM 6823=CIP 111902) and Asnod2-B07-B (RCC6908=KMM 6825=CIP 111904), respectively, as the type strains.
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http://dx.doi.org/10.1099/ijsem.0.004913 | DOI Listing |
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