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Single cell cloning of free-living amoebae with isolated square agar. | LitMetric

Single cell cloning of free-living amoebae with isolated square agar.

Parasitol Res

Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, 500, Porto Alegre, RS, 90050-170, Brazil.

Published: September 2021

AI Article Synopsis

  • An alternative method for cloning amoebas is introduced, which involves scraping cells from agar.
  • The cells are then placed in a new agar medium divided into isolated squares to prevent them from mixing.
  • This technique improves the reliability of cloning and allows for better quality control during the process.

Article Abstract

An alternative amoeba cloning technique is described. The amoebic cells were scraped from the agar and inoculated in a new agar medium separated in isolated squares, preventing a cell from invading the space of another cell, increasing the reliability of cloning, and providing an efficient quality control of technique.

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Source
http://dx.doi.org/10.1007/s00436-021-07275-9DOI Listing

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