AI Article Synopsis
- The study investigates the traditional process of preparing teeth for DNA extraction, which includes cleaning and pulverizing, to see how moisture affects DNA quality.
- Using qPCR, the researchers compared DNA yields from pig teeth with and without freeze-drying.
- The results indicated that freeze-drying significantly improved DNA yield while maintaining high DNA quality, suggesting it's a beneficial step in sample preparation.
Article Abstract
The common method of preparing teeth prior to DNA extraction involves cleaning, decontamination, drying and pulverisation. Moisture in post-mortem teeth can promote bacterial growth and hydrolytic damage that could contribute to DNA degradation, whilst also possibly reducing the efficiency of sample pulverisation and DNA release. Here we compared DNA extraction from pig teeth, with- and without freeze-drying, to examine the impact of removing moisture on DNA yield. Quantitative real-time polymerase chain reaction (qPCR) was used to quantify an 83 bp mitochondrial DNA fragment and two nuclear DNA fragments of 82 bp and 150 bp. The comparative results showed that sample preparation with freeze-drying resulted in a higher DNA yield without compromising the DNA quality. This study highlights the advantage of incorporating a freeze-drying to improve the DNA yield and minimising the loss of DNA during sample preparation of teeth.
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| http://dx.doi.org/10.1016/j.forsciint.2021.110938 | DOI Listing |
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