This protocol describes a method for the separation of radiolabeled oligonucleotides from unincorporated radiolabel that takes advantage of the reversible affinity of oligonucleotides for silica gel. This protocol can be used only to purify oligonucleotides carrying a 5'-phosphate group, radiolabeled or unlabeled.
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| http://dx.doi.org/10.1101/pdb.prot100727 | DOI Listing |
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