double mutants have a severely reduced brood size compared to the wild-type and compared to each single mutant. To determine if this low-fecundity phenotype is associated with oocyte maturity defects, we used markers to assess the maturity of oocytes in the proximal gonad. We studied phosphorylated histone H3, a marker normally associated with mature oocytes, and DAO-5, a nucleolar marker normally associated with immature oocytes. We found that in the double mutants, the oocyte occupying the -1 position frequently retains DAO-5 and fails to accumulate phosphorylated histone H3. This suggests that the simultaneous disruption of dynein and MEL-28 can lead to failure of the oocyte maturity program.
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| http://dx.doi.org/10.17912/micropub.biology.000421 | DOI Listing |
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