AI Article Synopsis
- The UltraPlex method enhances fluorescent immunohistochemistry by using hapten tags on primary antibodies, allowing for the simultaneous imaging of four or more antigens in a single tissue section.
- It employs anti-hapten secondary antibodies with fluorophores for amplified signal detection, and the process can be easily completed with standard fluorescence microscopy tools.
- This technique results in highly sensitive and specific staining, making it possible to visualize and analyze multiple cellular subtypes, such as tumor cells and infiltrating lymphocytes, and create composite images from multiple samples.
Article Abstract
The UltraPlex method for multiplexed two-dimensional fluorescent immunohistochemistry is described, in which hapten tags conjugated to primary antibodies facilitate multiplexed imaging of four or more antigens per tissue section at once. Anti-hapten secondary antibodies labeled with fluorophores provide amplified signal for detection, which is accomplished using a standard fluorescent microscope or digital slide scanner. The protocol is rapid and straightforward and utilizes conventionally prepared tissue samples. The resulting staining is highly sensitive and specific, enabling high-resolution imaging of multiple cellular subtypes within tissue samples. Tumor cells and tumor-infiltrating lymphocytes are presented as examples. Multiple 4-plex-stained tissue samples can be digitally overlaid to create 8-plex (or more) high-content images, enabling visualization of distribution of complex cellular subtypes across tissues.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11290477 | PMC |
| http://dx.doi.org/10.1007/978-1-0716-1593-5_17 | DOI Listing |
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