The dual-axis confocal (DAC) configuration provides a high axial resolution, long working distance (WD), and large dynamic range. These properties can reveal depth-resolved fluorescence spectra. We present a depth sensitive fluorescence spectroscopy based on the DAC configuration. The system enables high axial resolution of 3.23 µm and a long WD of 3.73 mm compared to that of 4.68 µm and 2.1 mm for comparable single-axis confocal configurations, respectively. Besides, a DAC configuration also offers a superior dynamic range and rejection of out-of-focus scattered light based on the principle of Huygens-Fresnel integrals. Additionally, to locate the target layer, the collection path of the DAC configuration will be used as the other illumination path, forming a dual-axis illumination configuration. These beam paths are used to locate the target layer using a white light imaging system with a commercial low numerical aperture objective. A multi-layer fluorescence phantom of Barrett's esophagus containing fluorescein isothiocyanate and Alexa Fluor 514 was used to verify the principle of depth-resolved fluorescence spectroscopy. The results show that the DAC configuration can collect fluorescence spectra from microscopic regions with high axial resolution.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/OL.428193 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Asymmetric Polarization Modulation of d-p Hybridization-Enhanced Bidirectional Sulfur Redox Kinetics with Heteronuclear Dual-Atom Catalysts.
ACS Nano
December 2024
Department of Materials Science and Engineering, National University of Singapore, Singapore 117574, Republic of Singapore.
Lithium sulfur batteries (LiSBs) represent a highly promising avenue for future energy storage systems, offering high energy density and eco-friendliness. However, the sluggish kinetics of the sulfur redox reaction (SRR) poses a significant challenge to their widespread applications. To tackle this challenge, we have developed an efficient heteronuclear dual-atom catalyst (hetero-DAC) that leverages surface charge polarization to enhance the asymmetric adsorption of sulfur intermediates.
View Article and Find Full Text PDFExploring Geometric Properties and Cycle Design in Packed Bed and Monolith Contactors Using Temperature-Vacuum Swing Adsorption Modeling for Direct Air Capture.
Ind Eng Chem Res
November 2024
Institute of Energy and Process Engineering, ETH Zurich, 8092 Zurich, Switzerland.
This study presents a comprehensive comparison between the packed bed and monolith contactor configurations for direct air capture (DAC) via process modeling of a temperature-vacuum swing adsorption (TVSA) process. We investigate various design parameters to optimize performance across different contactor geometries, including pellet size, monolith wall thickness, active sorbent content in monoliths, and packed bed structure configurations, considering both a traditional long column (PB) and multiple shorter columns configured in parallel (PB). Our parametric analysis assesses specific exergy consumption, sorbent, and volume requirements across different operating conditions of a five-step TVSA cycle.
View Article and Find Full Text PDFTheoretical study of a CuCo dual-atom catalyst for nitrogen fixation.
RSC Adv
October 2024
School of Science, Huzhou University Huzhou Zhejiang 313000 China
Developing low-cost catalysts for highly efficient nitrogen reduction reaction (NRR) in industrial applications is a great challenge. Dual-atom catalysts (DACs) have also aroused scientific interest as potential NRR catalysts due to their possible higher activity and atom utilization than single atom catalysts. Using density functional theory, we have investigated the NRR performances of heteronuclear CuCo DACs with different coordination configurations.
View Article and Find Full Text PDFHyperparameter optimization: Classics, acceleration, online, multi-objective, and tools.
Math Biosci Eng
June 2024
Department of Computer Science and Engineering, Shanghai Jiao Tong University, Shanghai, China.
Hyperparameter optimization (HPO) has been well-developed and evolved into a well-established research topic over the decades. With the success and wide application of deep learning, HPO has garnered increased attention, particularly within the realm of machine learning model training and inference. The primary objective is to mitigate the challenges associated with manual hyperparameter tuning, which can be ad-hoc, reliant on human expertise, and consequently hinders reproducibility while inflating deployment costs.
View Article and Find Full Text PDFCoupled Ni─Co Dual-Atom Catalyst for Guiding Sulfur and Lithium Evolutions in Lithium-Sulfur Batteries.
Small
November 2024
Institute of Applied Physics and Materials Engineering, University of Macau, Macao, SAR, 999078, China.
The kinetically retarded sulfur evolution reactions and notorious lithium dendrites as the major obstacles hamper the practical implementation of lithium-sulfur batteries (LSBs). Dual metal atom catalysts as a new model are expected to show higher activity by their rational coupling. Herein, the dual-atom catalyst with coupled Ni─Co atom pairs (Ni/Co-DAC) is designed successfully by programmed approaches.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!