AI Article Synopsis
- - The COVID-19 pandemic has caused significant harm globally, affecting families, healthcare, and economies, highlighting the need for effective diagnostics and treatments.
- - Researchers developed new DNA aptamers that specifically bind to the SARS-CoV-2 spike protein, demonstrating high binding strength and specificity.
- - The aptamer, named SNAP1, was successfully used in diagnostic tests to detect low levels of inactivated SARS-CoV-2, indicating its potential as a useful tool for diagnosing the virus.
Article Abstract
The coronavirus disease 2019 (COVID-19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for distinct viral proteins are critical components for rapid diagnostics and targeted therapeutics. In this work, we demonstrate the selection of novel DNA aptamers that bind to the SARS-CoV-2 spike glycoprotein with high specificity and affinity (<80 nM). Through binding assays and high resolution cryo-EM, we demonstrate that SNAP1 (SARS-CoV-2 spike protein N-terminal domain-binding aptamer 1) binds to the S N-terminal domain. We applied SNAP1 in lateral flow assays (LFAs) and ELISAs to detect UV-inactivated SARS-CoV-2 at concentrations as low as 5×10 copies mL . SNAP1 is therefore a promising molecular tool for SARS-CoV-2 diagnostics.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426805 | PMC |
| http://dx.doi.org/10.1002/anie.202107730 | DOI Listing |
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