AI Article Synopsis
- The study focused on the atomic makeup and protein profiles of saliva proteins that cling to orthodontic metallic brackets and hydroxyapatite (HA) discs.
- Researchers used X-ray Photoelectron Spectroscopy to analyze brackets and mass spectrometry to identify and characterize salivary proteins after immersion in human saliva.
- The results showed significant differences in the proteins adhering to each surface; 311 proteins were unique to HA discs, 253 were unique to metallic brackets, and only 45 proteins were found in both, with some related to antimicrobial activity and remineralization present in both groups.
Article Abstract
The aim of this study was to investigate the atomic composition and the proteome of the salivary proteins adsorbed on the surface of orthodontic metallic bracket. For this, the atomic composition of orthodontic metallic brackets was analyzed with X-ray Photoelectron Spectroscopy (XPS). The acquired bracket pellicle was characterized after brackets were immersed in human whole saliva supernatant for 2 hours at 37°C. Hydroxyapatite (HA) discs were used as a control. Acquired pellicle was harvested from the HA discs (n = 12) and from the metallic brackets (n = 12). Proteomics based on mass spectrometry technology was used for salivary protein identification and characterization. Results showed that most of the proteins adsorbed on the surface of orthodontic metallic brackets and on the HA discs were identified specifically to each group, indicating a small overlapping between the salivary proteins on each study group. A total of 311 proteins present on the HA discs were unique to this group while 253 proteins were unique to metallic brackets, and only 45 proteins were common to the two groups. Even though most proteins were unique to each study group, proteins related to antimicrobial activity, lubrication, and remineralization were present in both groups. These findings demonstrate that the salivary proteins adsorbed on the bracket surface are dependent on the material molecular composition.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318307 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254909 | PLOS |
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