Jinwu Jiangu Capsule is a medicinal formula from the Chinese Miao nationality. Leflunomide is recommended in organizational guidelines for the treatment of rheumatoid arthritis (RA). To investigate the effect of Jinwu Jiangu Capsule on PI3K/Akt/mTOR signal pathway in cells taken from RA patients New Zealand rabbits were administrated with Jinwu Jiangu Capsule suspension to prepare serum containing medicine. Lyophilized powder was prepared from this serum for cell treatment. The expression of LC3-II and PI3K, AKT, mTOR were detected by IF and western blot. Moreover, the levels of Atg1, Atg5, Atg14 were detected by RT-qPCR. The results showed that the expression of LC3-II was increased, and fluorescence spot of LC3-II was obvious in high-dose of Jinwu Jiangu Capsule group. Jinwu Jiangu Capsule decreased the level of PI3k, Akt, and mTOR protein, and increased the levels of Atg1, Atg5 and Atg14. Specially, the high-dose of Jinwu Jiangu Capsule had the most obvious inhibitory and up-regulation effects. However, there was no significant difference in the expression of Akt, mTOR and Atg1 in the medium-dose of Jinwu Jiangu Capsule group compared with the leflunomide group. In conclusion, Jinwu Jiangu Capsule regulates autophagy by inhibiting the PI3K/AKT/mTOR pathway in RA.
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Jinwu Jiangu capsule attenuates rheumatoid arthritis via the SLC7A11/GSH/GPX4 pathway in M1 macrophages.
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December 2024
Guizhou University of Traditional Chinese Medicine, Guiyang, 550000, China. Electronic address:
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- JWJGC is a Chinese herbal medicine that has been shown to alleviate symptoms of rheumatoid arthritis (RA) by reducing reactive oxygen species (ROS) and modulating macrophage activity.
- In a study with rats suffering from collagen-induced arthritis, JWJGC improved joint swelling, restored the M1/M2 macrophage balance, and decreased inflammatory markers.
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Jinwu Jiangu Capsule alleviates rheumatoid arthritis symptoms by regulating the ADCY10 and cAMP/RANKL pathways.
J Ethnopharmacol
February 2025
Department of Rheumatology Immunology, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou Province, 550001, China; Department of Liupanshui Hospital of Traditional Chinese Medicine, Liupanshui, Guizhou Province, 553000, China. Electronic address:
Ethnopharmacological Relevance: Rheumatoid arthritis (RA) is a systemic autoimmune disease that primarily affects joints and damages other tissues, including the heart, kidneys, lungs, digestive system, eyes, skin and nervous system. Jinwu Jiangu Capsule (JWJG) is effective in tonifying kidneys, activating blood circulation, and dispelling wind and dampness. Meanwhile, it produces favorable clinical results in relieving joint pain and reducing inflammation.
View Article and Find Full Text PDFJinwu Jiangu Capsule affects synovial cells in rheumatoid arthritis through PI3K/Akt/mTOR signaling pathway.
Acta Biochim Pol
July 2021
Department of Rheumatology and Immunology, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou Province, 550001, China.
Jinwu Jiangu Capsule is a medicinal formula from the Chinese Miao nationality. Leflunomide is recommended in organizational guidelines for the treatment of rheumatoid arthritis (RA). To investigate the effect of Jinwu Jiangu Capsule on PI3K/Akt/mTOR signal pathway in cells taken from RA patients New Zealand rabbits were administrated with Jinwu Jiangu Capsule suspension to prepare serum containing medicine.
View Article and Find Full Text PDF[Effects of Jinwu Jiangu recipe on IL-17/STAT3 signals in rheumatoid arthritis synoviocytes].
Zhongguo Zhong Yao Za Zhi
February 2018
Article Synopsis
- The study investigated how the Jinwu Jiangu recipe total extract affects IL-17/STAT3 signaling in rheumatoid arthritis synovial fibroblasts (RASF).
- The primary RASFs were treated with varying doses of the extract and compared to control groups, analyzing gene expression and protein activity through PCR and Western blot assays.
- Results indicated that the Jinwu Jiangu extract significantly reduced mRNA and protein levels of IL-17R and pSTAT3, particularly at higher doses, suggesting its potential therapeutic effects on rheumatoid arthritis.
[Effect of Jinwu Jiangu Recipe on Expressions of NF-kappaB and IL-17 in Collagen Induced Arthritis Model Rats].
Zhongguo Zhong Xi Yi Jie He Za Zhi
May 2016
Objective: To explore the effect of Jinwu Jiangu Recipe (JJR) on the expression of synovial cells' nuclear factor-kappaB (NF-kappaB) and serum interleukin 17 (IL-17) in collagen induced arthritis (CIA) rats.
Methods: Totally 60 Wistar rats were randomly divided into 6 groups, i.e.
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