AI Article Synopsis
- Researchers isolated natural killer (NK) cells from healthy newborn umbilical cord blood and reprogrammed them using specific genetic factors delivered through nucleofection.
- The resulting cell line, named MUSIi013-A, showed high expression of markers associated with pluripotency and an ability to differentiate into all three germ layers, while also maintaining a normal chromosome structure.
- This iPSC line could be significant for studying epigenetic memory, which may influence NK cell differentiation in induced pluripotent stem cells (hiPSCs).
Article Abstract
Natural killer (NK) cells were isolated from human umbilical cord blood from a healthy newborn and reprogrammed by episomal vectors carrying reprograming factors L-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53 delivered using nucleofection. The obtained MUSIi013-A human induced pluripotent stem cell (iPSC) line highly expressed pluripotency markers, had the capacity to differentiate into derivatives of the three germ layers, while retained a normal karyotype. This cell line may be a useful tool to study epigenic memory that may predispose hiPSCs to enhanced NK differentiation.
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| http://dx.doi.org/10.1016/j.scr.2021.102472 | DOI Listing |
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